Amino acid analysis (aaa) determines the amount of protein in a sample and the composition, i.e. ratios between the different amino acids. Aaa is the only method for determination of the absolute amount of a protein. The composition, presented as mol-% can be used for identification of a protein from databases.
The analyses consist of chromatographic separation of all amino acids in the protein and quantification of each of them. For proteins and peptides this separation requires hydrolysis of peptide bonds to obtain the free amino acids.
Contacts
Technician Anne Blicher (daily at 9-15)
Associate Professor Susanne Jacobsen